Bacterial Acetone Carboxylase Is a Manganese-dependent Metalloenzyme

Bacterial acetone carboxylase is a manganese-dependent metalloenzyme.

Bacterial acetone carboxylase catalyzes the ATP-dependent carboxylation of acetone to acetoacetate with the concomitant production of AMP and two inorganic phosphates. The importance of manganese in Rhodobacter capsulatus acetone carboxylase has been established through a combination of physiological, biochemical, and spectroscopic studies. Depletion of manganese from the R. capsulatus growth m...

Nitrate-dependent degradation of acetone by Alicycliphilus and Paracoccus strains and comparison of acetone carboxylase enzymes.

A novel acetone-degrading, nitrate-reducing bacterium, strain KN Bun08, was isolated from an enrichment culture with butanone and nitrate as the sole sources of carbon and energy. The cells were motile short rods, 0.5 to 1 by 1 to 2 μm in size, which gave Gram-positive staining results in the exponential growth phase and Gram-negative staining results in the stationary-growth phase. Based on 16...

Evidence for an inducible nucleotide-dependent acetone carboxylase in Rhodococcus rhodochrous B276.

The metabolism of acetone was investigated in the actinomycete Rhodococcus rhodochrous (formerly Nocardia corallina) B276. Suspensions of acetone- and isopropanol-grown R. rhodochrous readily metabolized acetone. In contrast, R. rhodochrous cells cultured with glucose as the carbon source lacked the ability to metabolize acetone at the onset of the assay but gained the ability to do so in a tim...

Pyruvate carboxylase. VII. A possible role for tightly bound manganese.

Acetone oxidation using ozone on manganese oxide catalysts.

Supported manganese oxide catalysts were prepared by the impregnation of alumina foam blocks washcoated with alumina and silica. The manganese content based on the weight of the washcoats was 10 wt % calculated as MnO2. Fourier transform profiles of the Mn K-edge EXAFS spectra for these samples gave three distinctive peaks at 0.15, 0.25, and 0.32 nm and were close to the profiles of Mn3O4 and b...